Disinfectant wipes are appropriate to control microbial bioburden from surfaces: use of a new ASTM standard test

S A Sattar1, C Bradley2, R Kibbee1

  • 1Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.

Abstract

Insights

Disinfectant pre-soaked wipes (DPW) effectively reduce bacteria on surfaces. However, a new standard shows only one tested wipe prevented bacterial transfer to clean surfaces, highlighting the need for improved wipe efficacy testing.

Area of Science:

  • Microbiology
  • Infection Control
  • Environmental Hygiene

Background:

  • Disinfectant pre-soaked wipes (DPW) are increasingly used for high-touch environmental surfaces (HTES) in healthcare.
  • Current testing methods rarely simulate field use conditions for DPW.
  • Label claims for disinfectants often omit wiping action efficacy.

Purpose of the Study:

  • Evaluate the new ASTM E2967-15 standard for wipe testing.
  • Assess DPW efficacy in decontaminating surfaces.
  • Determine the capacity of DPW to transfer acquired contamination.

Main Methods:

  • Utilized ASTM Standard E2967-15 across three independent laboratories.
  • Tested five commercially available DPW products.
  • Analyzed data for reproducibility and repeatability of the standard.

Main Results:

  • All tested DPW achieved >4log10 reduction of Staphylococcus aureus and Acinetobacter baumanii with 10s wiping.
  • Only one DPW, containing 0.5% accelerated H2O2, prevented bacterial transfer to a clean surface.
  • Significant variability in bacterial reduction and transfer prevention was observed among products.

Conclusions:

  • The new ASTM E2967-15 standard is a significant advancement for assessing DPW microbial decontamination of HTES.
  • This standard will aid research and development for more reliable DPW claims.
  • Further research is needed to optimize DPW formulations for both surface decontamination and prevention of microbial transfer.

Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
2.0K
Surface Membrane Barriers01:18

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
3.3K
Cleaning, Sterilization, and Disinfection01:30

Cleaning, Sterilization, and Disinfection

Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
13.4K
Chemical Agents for Microbial Control01:27

Chemical Agents for Microbial Control

Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
1.5K
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
40
Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
25.4K