Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

835
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
835
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

653
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
653
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.8K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Candidemia: the most frequent invasive fungal infection in France].

La Revue du praticien·2026
Same author

Antifungal exposure in <i>Scedosporium</i> septic arthritis: synovial penetration of voriconazole and terbinafine and plasma concentrations of olorofim.

Antimicrobial agents and chemotherapy·2026
Same author

Overlooked risk factors for European autochthonous strongyloidiasis, Paris, France.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Pneumocystis jirovecii pneumonia in solid organ transplant recipients: a prospective observational cohort study.

The Journal of infection·2026
Same author

Endocarditis Due to <i>Scedosporium</i> spp and <i>Lomentospora prolificans</i>: Case Series and Literature Review.

Open forum infectious diseases·2026
Same author

Assessment of the feed additive consisting of 6-phytase (4a23) (produced with <i>Komagataella phaffii</i>DSM 25375) (ENZY PHOSTAR<sup>®</sup> P/L) for all poultry species, weaned piglets, pigs for fattening, sows and minor porcine species for the renewal of its authorisation (Kaesler Nutrition GmbH).

EFSA journal. European Food Safety Authority·2026

Related Experiment Video

Updated: Mar 31, 2026

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
08:45

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

14.3K

Typing Candida Species Using Microsatellite Length Polymorphism and Multilocus Sequence Typing.

Dea Garcia-Hermoso1,2, Marie Desnos-Ollivier1,2, Stéphane Bretagne3,4,5

  • 1Institut Pasteur, Unité de Mycologie Moléculaire, Centre National de Référence Mycoses Invasives et Antifongiques, 25 rue du Dr. Roux, 75724, Paris cedex 15, France.

Methods in Molecular Biology (Clifton, N.J.)
|November 1, 2015
PubMed
Summary

Two molecular typing methods, microsatellite length polymorphism (MLP) and multilocus sequence typing (MLST), aid in understanding epidemiological links between Candida species isolates. Both offer distinct advantages and disadvantages for fungal isolate genetic relatedness studies.

Keywords:
Candida albicansCandida glabrataCandida kruseiCandida parapsilosisCandida tropicalisGenotypingITS sequencingMicrosatellite polymorphic markerMold identificationMultilocus sequence typingNon-sporulating mold sRespiratory specimen sShort tandem repeat

More Related Videos

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

7.4K
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

12.4K

Related Experiment Videos

Last Updated: Mar 31, 2026

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
08:45

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

14.3K
Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

7.4K
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

12.4K

Area of Science:

  • Medical Mycology
  • Molecular Epidemiology
  • Genetics

Background:

  • Understanding epidemiological relationships between Candida spp. isolates is crucial.
  • Several molecular typing techniques have been developed for this purpose.

Purpose of the Study:

  • To compare two prominent molecular typing methods: microsatellite length polymorphism (MLP) and multilocus sequence typing (MLST).
  • To evaluate their utility in discerning epidemiological relationships among Candida spp. isolates.

Main Methods:

  • Microsatellite Length Polymorphism (MLP): Involves in silico selection of repetitive DNA sequences, amplification with labeled primers, and fragment sizing via electrophoresis.
  • Multilocus Sequence Typing (MLST): Utilizes Sanger sequencing to identify single-nucleotide polymorphisms within housekeeping genes.
  • Both methods employ specific amplifications with stringent, reproducible conditions.

Main Results:

  • MLST provides easily comparable results between laboratories, with publicly available schemes for key Candida species.
  • MLP requires standardization for inter-platform comparability due to electrophoretic variations.
  • Both MLP and MLST offer insights into fungal isolate genetic relatedness, each with specific strengths and limitations.

Conclusions:

  • The choice between MLP and MLST depends on the specific research objective.
  • Both methods are valuable tools for investigating the genetic relatedness of Candida spp. isolates.
  • Advancements in genomic knowledge facilitated the development of these methods in the 2000s.