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Implementation of automated testing for 1,25-dihydroxyvitamin D: Return of experience from a core-laboratory
Nathalie Miller1, Damien Gruson2
1Department of Laboratory Medicine, Cliniques Universitaires St-Luc, Brussels, Belgium.
Abstract:
Measurement of 1,25(OH)2D, the most biologically active form of vitamin D, circulating levels is relevant in several physiopathological states such as chronic kidney disease, parathyroid dysfunction, sarcoidosis, and vitamin D dependent rickets. Our study determined the performances of a novel automated 1,25(OH)2D immunoassay in a core-laboratory environment. We observed satisfactory analytical performances for this assay and an excellent agreement with a well established LC-MS/MS method. Furthermore, this assay allows a reduced TAT, integration in automated core-laboratories and potential consolidation with other tests of the bone and mineral metabolism.
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