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Related Concept Videos

Proteomics01:33

Proteomics

10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Updated: Mar 31, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Introduction to Proteomics Technologies.

Christof Lenz1,2, Hassan Dihazi3

  • 1Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 1, 2015
PubMed
Summary
This summary is machine-generated.

Proteomics mass spectrometry is an emerging technology, now powerful and accessible for quantitative analysis of whole proteomes. This enables feasible global comparative proteome studies.

Keywords:
2-DEElectrophoresisMass spectrometryProteomicsSeparations

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Proteomics is an emerging field compared to genomics and transcriptomics.
  • Significant advancements have made proteomics technology more powerful, streamlined, and accessible.
  • Landmark studies in 2014 provided the first draft versions of the human proteome.

Purpose of the Study:

  • To introduce the background of mass spectrometry (MS)-based proteomics.
  • To explain the core concepts of MS-based proteomics and protein/peptide separations.
  • To focus on the properties of datasets generated from these studies.

Main Methods:

  • Mass spectrometry (MS) as a core technology in proteomics.
  • Integration with advanced separation techniques (e.g., chromatography).
  • Utilizing sophisticated data processing and bioinformatics solutions.

Main Results:

  • MS-based proteomics enables quantitative analysis of whole proteomes.
  • Global comparative proteome studies are now feasible within days.
  • Understanding of protein and peptide separation properties is crucial.

Conclusions:

  • Mass spectrometry is central to modern proteomics.
  • Technological maturity is increasing, making proteomics more accessible.
  • The field is rapidly advancing, enabling large-scale proteome analysis.