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Updated: Mar 31, 2026

Biobank for Translational Medicine: Standard Operating Procedures for Optimal Sample Management

Biobank for Translational Medicine: Standard Operating Procedures for Optimal Sample Management

Published on: November 30, 2022

Using GenBank.

Eric W Sayers¹, Ilene Karsch-Mizrachi²

¹National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Building 45, 45 Center Drive, Bethesda, MD, 20892, USA. sayers@ncbi.nlm.nih.gov.

Methods in Molecular Biology (Clifton, N.J.)

|November 1, 2015

Summary

This summary is machine-generated.

GenBank is a vital DNA sequence database covering 300,000 organisms, including over 110,000 plants. It ensures global data coverage through daily exchanges with international archives and offers integrated analysis tools.

Keywords:

BLAST DNA Sequence Entrez MegaBLAST NCBI

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Area of Science:

Bioinformatics
Genomics
Molecular Biology

Background:

GenBank is a primary repository for publicly available DNA sequences.
It houses data from numerous organisms, with a significant portion from embryophyta (plants).
Data is sourced from individual labs and large-scale sequencing initiatives.

Purpose of the Study:

To describe the GenBank database and its functionalities.
To highlight its integration with other biological data resources.
To inform users about data access and analysis methods.

Main Methods:

Data compilation from global submissions and international archives (ENA, DDBJ).
Integration with the NCBI Entrez system for cross-database retrieval.
Utilizing BLAST for sequence similarity searches.
Providing data via FTP and web-based NCBI tools.

Main Results:

GenBank contains sequences from 300,000 named organisms, including over 110,000 plant species.
Worldwide data coverage is achieved through daily synchronization with ENA and DDBJ.
NCBI Entrez provides integrated access to sequence, genome, and literature data.
BLAST enables efficient sequence similarity searches.

Conclusions:

GenBank is an essential, comprehensive resource for genomic information.
Its integration with NCBI tools facilitates diverse research applications.
Accessible data and analysis services support a wide range of scientific inquiries.