Electroblotting from Polyacrylamide Gels
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Updated: Mar 30, 2026

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Aaron Goldman1, Jeanine A Ursitti1, Jacek Mozdzanowski1
1The Wistar Institute, Philadelphia, Pennsylvania.
Abstract:
Transferring proteins from polyacrylamide gels onto retentive membranes is now primarily used for immunoblotting. A second application that was quite common up to about a decade ago was electroblotting of proteins for N-terminal and internal sequencing using Edman chemistry. This unit contains procedures for electroblotting proteins from polyacrylamide gels onto a variety of membranes, including polyvinylidene difluoride (PVDF) and nitrocellulose. In addition to the commonly used tank or wet transfer system, protocols are provided for electroblotting using semidry and dry systems. This unit also describes procedures for eluting proteins from membranes using detergents or acidic extraction with organic solvents for specialized applications.
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