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Does glucose decrease cytosolic free calcium in normal pancreatic islet cells?

P Gobbe1, A Herchuelz

  • 1Laboratory of Pharmacology, Brussels University School of Medicine, Belgium.

Research Communications in Chemical Pathology and Pharmacology
|February 1, 1989
PubMed
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Glucose affects cytosolic free calcium concentration ([Ca2+]i) in rat pancreatic islet cells. It initially decreases [Ca2+]i before causing a secondary rise, suggesting dual regulatory effects.

Area of Science:

  • Endocrinology
  • Cell Physiology
  • Calcium Signaling

Background:

  • Cytosolic free calcium concentration ([Ca2+]i) is crucial for pancreatic islet cell function.
  • Glucose metabolism regulates insulin secretion and intracellular calcium dynamics.

Purpose of the Study:

  • To investigate the dynamic changes in [Ca2+]i in response to glucose in normal rat pancreatic islet cells.
  • To elucidate the mechanisms underlying glucose-induced alterations in [Ca2+]i.

Main Methods:

  • Measurement of [Ca2+]i using the fura 2 intracellular calcium indicator.
  • Utilizing isolated normal rat pancreatic islet cells.

Main Results:

  • Glucose induced a biphasic response in [Ca2+]i: an initial decrease followed by a secondary rise.

Related Experiment Videos

  • The initial decrease in [Ca2+]i mirrored glucose-induced 45Ca efflux.
  • The secondary rise in [Ca2+]i was attributed to increased calcium influx.

    • Conclusions:

    • Glucose exerts a dual effect on [Ca2+]i in pancreatic islet cells.
    • Glucose may inhibit and stimulate [Ca2+]i through distinct mechanisms.
    • These findings contribute to understanding glucose-mediated calcium signaling in islets.