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Related Experiment Video

Updated: Mar 30, 2026

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

Two-dimensional single-cell patterning with one cell per well driven by surface acoustic waves.

David J Collins¹, Belinda Morahan², Jose Garcia-Bustos²

¹Department of Mechanical and Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia.

Nature Communications

|November 3, 2015

Summary

This summary is machine-generated.

Researchers developed a novel acoustic field method for precisely patterning single cells and particles. This reusable technique enables detailed single-cell analysis, overcoming limitations of previous disposable methods.

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Last Updated: Mar 30, 2026

Cell Co-culture Patterning Using Aqueous Two-phase Systems

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Published on: March 26, 2013

Author Spotlight: Development of a Scaffold-Free Acoustic Assembly Method for High-Quality 3D Cell Spheroid Culture

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A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

Area of Science:

Biotechnology
Cell Biology
Acoustic Physics

Background:

Single-cell analysis requires precise trapping and patterning of individual cells for temporal studies.
Existing mechanical methods for cell patterning are effective but typically result in single-use devices.

Purpose of the Study:

To introduce a new, reusable method for spatially separating and patterning single particles and cells.
To demonstrate the application of high-frequency acoustic fields for single-cell manipulation.

Main Methods:

Utilizing high-frequency acoustic fields to create "acoustic wells" for individual cell confinement.
Operating within an unexplored acoustic regime where wavelength is comparable to cell dimensions.
Characterizing the patterning of various particle sizes and specific cell types, including human lymphocytes and infected red blood cells.

Main Results:

Successful patterning of multiple spatially separated single particles and cells.
Demonstrated capture and patterning of human lymphocytes and Plasmodium falciparum-infected red blood cells.
Validated the efficacy of the acoustic field method across a range of particle sizes.

Conclusions:

High-frequency acoustic fields offer a versatile and reusable platform for single-cell patterning.
This technique advances the capabilities of single-cell analysis by enabling precise, non-destructive cell manipulation.
The method holds potential for applications in diagnostics and drug discovery involving individual cell behavior.