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Selectable Surface and Bulk Fluorescence Imaging with Plasmon-Coupled Waveguides.

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This study introduces a novel fluorescence imaging method using a plasmon waveguide to selectively visualize surface-bound probes or simultaneous surface and bulk fluorescence. This technique offers independent control over imaging specific regions for enhanced chemical and biological analysis.

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Area of Science:

  • Optics and Photonics
  • Surface Science
  • Biophysical Chemistry

Background:

  • Selective fluorescence imaging is crucial for analyzing molecular interactions at interfaces.
  • Distinguishing between surface-bound and bulk solution fluorescence is challenging with conventional methods.
  • Plasmon waveguides offer unique optical properties for nanoscale light manipulation.

Purpose of the Study:

  • To develop a new method for selective imaging of surface-bound probes.
  • To enable simultaneous imaging of surface-bound and bulk solution fluorescence.
  • To provide independent control over surface and bulk fluorescence detection.

Main Methods:

  • Utilizing two optical modes with distinct properties within a plasmon waveguide.
  • Exploiting differential coupling of modes to fluorophores at varying distances from the interface.
  • Employing electric-driven liquid crystal in back focal plane (BFP) imaging for selective detection.
  • Analyzing coupled emission rings with different polarizations and emitting angles in BFP images.

Main Results:

  • Demonstrated selective imaging of surface-bound probes.
  • Achieved simultaneous imaging of surface-bound and bulk fluorescence.
  • Showcased independent switching (ON/OFF) of surface and bulk fluorescence signals.
  • Confirmed distinct polarization states and emitting angles for coupled emissions.

Conclusions:

  • A novel, versatile fluorescence imaging technique based on plasmon waveguides has been established.
  • The method allows for selective or simultaneous detection of surface and bulk fluorescence.
  • This planar multilayer film approach has broad applicability in chemistry, materials science, molecular biology, and medicine.
  • The independent control over fluorescence signals enhances the utility of fluorescence-based techniques.