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Analysis of Imprinted Gene Regulation
David A Skaar1, Randy L Jirtle2,3
1Department of Biological Sciences, North Carolina State University, Raleigh, NC, 27695, USA. daskaar@ncsu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|November 4, 2015
Summary
Epigenetic studies can now identify gene variations linked to traits. This research focuses on imprinted genes, examining DNA methylation and parent-specific gene expression to understand their role in development.
Area of Science:
- Epigenetics
- Developmental Biology
- Genetics
Background:
- Genetic studies identify sequence variants linked to phenotypes.
- Epigenetics, the study of gene expression regulation, is a growing field.
- Imprinted genes, crucial for early development, exhibit parent-specific regulation.
Purpose of the Study:
- To explore associations between epigenetic variation and phenotypes.
- To describe methods for studying epigenetic regulation in imprinted genes.
Main Methods:
- Examining differentially methylated regions (DMRs) in imprinted genes.
- Analyzing monoallelic expression patterns of imprinted genes.
Main Results:
- Methods are described for assessing allele-specific methylation in DMRs.
- Techniques for evaluating parent-of-origin specific gene silencing and transcription are detailed.
Conclusions:
- Epigenetic variation, particularly in imprinted genes, can be studied using DMRs and monoallelic expression.
- These methods provide insights into the epigenetic regulation of gene expression and its role in development.
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