Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tunable electrostatic interactions of lipid-coated quantum dots with biological membranes.

bioRxiv : the preprint server for biology·2026
Same author

Iterative immunoprecipitation and phage pre-wash dramatically improve epitope-resolved serology by VirScan.

Frontiers in virology (Lausanne, Switzerland)·2026
Same author

Proximity proteomics reveals a role for IFI16 during human coronavirus infection.

bioRxiv : the preprint server for biology·2026
Same author

PABPC1 Modulates Immunoglobulin pre-mRNA Alternative Polyadenylation.

bioRxiv : the preprint server for biology·2026
Same author

Quantitative Mapping of Nanoscale EGFR-Grb2 Assemblies by DNA-PAINT.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Wash-Free Multi-Target Super-Resolution Microscopy With Photocaged DNA Labels.

Angewandte Chemie (International ed. in English)·2026
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: Mar 30, 2026

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

12.4K

Sample Preparation and Data Acquisition for μs-ALEX.

Achillefs Kapanidis, Devdoot Majumdar, Mike Heilemann

    Cold Spring Harbor Protocols
    |November 4, 2015
    PubMed
    Summary
    This summary is machine-generated.

    This protocol details sample preparation and data acquisition for microsecond-alternating laser excitation. Proper sample dilution is crucial for accurate single-event detection in experiments.

    More Related Videos

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
    07:55

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

    Published on: September 22, 2017

    10.8K
    Laser Micro-Irradiation to Study DNA Recruitment During S Phase
    07:11

    Laser Micro-Irradiation to Study DNA Recruitment During S Phase

    Published on: April 16, 2021

    5.0K

    Related Experiment Videos

    Last Updated: Mar 30, 2026

    Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
    08:48

    Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

    Published on: September 5, 2012

    12.4K
    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
    07:55

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

    Published on: September 22, 2017

    10.8K
    Laser Micro-Irradiation to Study DNA Recruitment During S Phase
    07:11

    Laser Micro-Irradiation to Study DNA Recruitment During S Phase

    Published on: April 16, 2021

    5.0K

    Area of Science:

    • Spectroscopy and Photochemistry
    • Biophysical Techniques

    Background:

    • Microsecond-alternating laser excitation is a powerful technique for studying dynamic processes.
    • Accurate data acquisition is essential for reliable results in laser-based experiments.

    Purpose of the Study:

    • To provide a detailed protocol for sample preparation and data acquisition.
    • To ensure reliable and reproducible results using microsecond-alternating laser excitation.

    Main Methods:

    • Sample preparation involves precise dilution to achieve single-event detection.
    • Data acquisition protocols are optimized for microsecond-alternating laser excitation.

    Main Results:

    • The protocol enables the successful preparation of samples for laser excitation.
    • Data acquisition parameters are established for effective signal capture.

    Conclusions:

    • This protocol standardizes sample preparation and data acquisition for microsecond-alternating laser excitation.
    • Adherence to this protocol facilitates high-quality data for scientific investigation.