Roseomonas eburnea sp. nov., isolated from activated sludge

Area of Science:

• Environmental microbiology
• Bacterial taxonomy
• Wastewater treatment microbiology

Background:

• The genus Roseomonas comprises Gram-negative bacteria with diverse metabolic capabilities.
• Microbial communities in industrial wastewater treatment facilities are crucial for pollutant degradation.
• Isolation and characterization of novel bacterial species contribute to understanding microbial diversity and function.

Purpose of the Study:

• To isolate and characterize a novel bacterial strain from an industrial wastewater treatment system.
• To determine the taxonomic position of the novel isolate using polyphasic characterization.
• To propose a new species within the genus Roseomonas.

Main Methods:

• Isolation of strain BUT-5T from activated sludge of an herbicide manufacturing wastewater treatment facility.
• Characterization of phenotypic, chemotaxonomic (fatty acids, respiratory quinones, polar lipids), and genotypic (16S rRNA gene sequencing, DNA-DNA hybridization) properties.
• Phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA relatedness values.

Main Results:

• Strain BUT-5T is a Gram-stain-negative, aerobic, short rod-shaped, non-endospore-forming, ivory-pigmented, and non-motile bacterium.
• Chemotaxonomic analysis revealed major fatty acids C16:0, C18:1 2-OH, summed feature 8, and ubiquinone Q-10 as the predominant respiratory quinone.
• Phylogenetic analysis showed highest 16S rRNA gene similarity to Roseomonas soli (97.5%), but low DNA-DNA relatedness (<46%) to related Roseomonas species, supporting its status as a new species.

Conclusions:

• Strain BUT-5T represents a novel species of the genus Roseomonas, named Roseomonas eburnea sp. nov.
• The proposed species, Roseomonas eburnea sp. nov., is distinct from previously described Roseomonas species based on genotypic and phenotypic data.
• This finding highlights the potential for discovering novel microorganisms in specialized industrial environments like wastewater treatment facilities.