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Updated: Mar 30, 2026
Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
Published on: January 2, 2016
TRPC1 is required for survival and proliferation of cochlear spiral ganglion stem/progenitor cells
Hsin-Chien Chen1, Chih-Hung Wang1, Cheng-Ping Shih1
1Department of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Objective:
The present studies were designed to test the hypothesis that canonical transient receptor potential channel 1 (TRPC1) is required for the proliferation of cochlear spiral ganglion stem/progenitor cells (SPCs).
Methods And Materials:
TRPC1 were detected and evaluated in postnatal day 1 CBA/CaJ mice pups derived-cochlear spiral ganglion SPCs by reverse transcription-polymerase chain reaction, Western blot, immunocytochemistry, and calcium imaging. The cell viability and proliferation of the spiral ganglion SPCs following si-RNA mediated knockdown of TRPC1 or addition of TRPC channel blocker SKF9635 were compared to controls.
Results:
In spiral ganglion SPCs, TRPC1 was found to be the most abundantly expressed TRPC subunit and shown to contribute to store-operated calcium entry. Silencing of TRPC1 or addition of TRPC channel blockers significantly decreased the rate of cell proliferation.
Conclusion:
The results suggest that TRPC1 might serve as an essential molecule in regulating the proliferation of spiral ganglion SPCs.
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