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Bioluminescence assay for cell viability.

G Yu Lomakina1, Yu A Modestova, N N Ugarova

  • 1Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia. nugarova@gmail.com.

Biochemistry. Biokhimiia
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Summary

The adenosine triphosphate bioluminescence assay, using firefly luciferin-luciferase, measures cell viability and microbial activity. This versatile method analyzes biological processes, metabolism, and cell death in various scientific fields.

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Area of Science:

  • Biochemistry
  • Microbiology
  • Cell Biology

Background:

  • The firefly luciferin-luciferase system provides a sensitive method for detecting adenosine triphosphate (ATP).
  • ATP is a universal indicator of metabolic activity and cellular viability.
  • Bioluminescence assays offer a rapid and quantitative approach to assess biological samples.

Purpose of the Study:

  • To review the theoretical underpinnings of the ATP bioluminescence assay.
  • To explore the diverse applications of this assay in microbiology, sanitation, medicine, and ecology.
  • To detail the assay's capability in analyzing microbial cultures and cellular processes.

Main Methods:

  • Utilizing the firefly luciferin-luciferase system for ATP detection.
  • Applying the assay to assess cell viability in various biological contexts.
  • Analyzing individual and mixed microbial cultures.
  • Investigating cellular processes such as necrosis and apoptosis.
  • Monitoring the dynamics of cellular metabolism.

Main Results:

  • The ATP bioluminescence assay is theoretically sound and widely applicable.
  • The method effectively assesses cell viability across different disciplines.
  • Diverse approaches exist for analyzing microbial populations using this assay.
  • The assay can reveal insights into cell death pathways and metabolic changes.

Conclusions:

  • The adenosine triphosphate bioluminescence assay is a powerful tool for biological research.
  • Its applications span from microbial analysis to understanding complex cellular events.
  • The method's versatility makes it invaluable in microbiology, medicine, and environmental science.