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A novel endolysin disrupts Streptococcus suis with high efficiency.

Wenhui Ji1, Qingqing Huang1, Liang Sun2

  • 1Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai 200240, China.

FEMS Microbiology Letters
|November 5, 2015
PubMed
Summary
This summary is machine-generated.

A novel phage-derived endolysin, Ly7917, shows broad lytic activity against drug-resistant Streptococcus suis 2. This endolysin effectively protected mice from virulent S. suis 2 infections, suggesting its therapeutic potential.

Keywords:
S. suisendolysinlytic activityprophage

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Area of Science:

  • Microbiology
  • Bacteriology
  • Antimicrobial Research

Background:

  • Streptococcus suis serotype 2 (S. suis 2) is a zoonotic pathogen causing significant human and swine industry losses due to antibiotic resistance.
  • The need for alternative therapies against multi-drug resistant S. suis 2 is critical.

Purpose of the Study:

  • To identify and characterize a novel endolysin with lytic activity against S. suis 2.
  • To evaluate the therapeutic efficacy of the endolysin Ly7917 against virulent S. suis 2 infections in a mouse model.

Main Methods:

  • Identification and purification of endolysin Ly7917 from S. suis temperate phage phi7917.
  • Characterization of Ly7917's lytic activity spectrum and optimal conditions (pH, Ca2+).
  • In vivo efficacy study using a murine model challenged with virulent S. suis 2 strain HA9801.

Main Results:

  • Ly7917 demonstrated broad lytic activity against S. suis types 1, 2, 7, and 9.
  • The endolysin retained activity at high pH and was enhanced by Ca2+.
  • Ly7917 treatment protected 90% of mice from lethal S. suis 2 challenge and reduced bacterial load by approximately 50%.

Conclusions:

  • Ly7917 is a potent endolysin with broad lytic activity against S. suis.
  • Ly7917 exhibits significant therapeutic potential as an alternative agent for treating infections caused by virulent S. suis strains.