Antibodies from combinatorial libraries use functional receptor pleiotropism to regulate cell fates
Richard A Lerner1, Rajesh K Grover1, Hongkai Zhang1
1Department of Cell and Molecular Biology,The Scripps Research Institute,La Jolla,CA 92037,USA.
Quarterly Reviews of Biophysics
|November 6, 2015
Summary
New antibody selection methods enable functional screening in animal cells, revealing insights into signal transduction. Some antibodies induce distinct cell fates compared to natural agonists, highlighting receptor functional pleiotropy.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Traditional antibody selection from combinatorial libraries focuses solely on binding affinity.
- Advancements in technology now permit functional selection of antibodies within cellular environments.
Purpose of the Study:
- To explore the functional selection of agonist antibodies in animal cells.
- To investigate novel insights into signal transduction pathways.
- To understand the implications of receptor functional pleiotropy.
Main Methods:
- Utilizing new methods for functional antibody selection in animal cells.
- Employing combinatorial libraries for antibody discovery.
- Analyzing antibody-mediated effects on cell fate and signal transduction.
Main Results:
- Selected agonist antibodies provide new perspectives on signal transduction.
- Certain antibodies binding to a receptor induce a different cell fate than the natural agonist.
- Demonstration of functional pleiotropy in receptor signaling.
Conclusions:
- Functional antibody selection offers a powerful tool for studying signal transduction.
- Receptor functional pleiotropy is a significant biological phenomenon.
- This approach can yield novel insights into complex cellular processes.
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