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Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
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Antibodies from combinatorial libraries use functional receptor pleiotropism to regulate cell fates

Richard A Lerner1, Rajesh K Grover1, Hongkai Zhang1

  • 1Department of Cell and Molecular Biology,The Scripps Research Institute,La Jolla,CA 92037,USA.

Quarterly Reviews of Biophysics
|November 6, 2015
PubMed

To date, most antibodies from combinatorial libraries have been selected purely on the basis of binding. However, new methods now allow selection on the basis of function in animal cells. These selected agonist antibodies have given new insights into the important problem of signal transduction. Remarkably, when some antibodies bind to a given receptor they induce a cell fate that is different than that induced by the natural agonist to the same receptor. The fact that receptors can be functionally pleiotropic may yield new insights into the important problem of signal transduction.

Keywords:
Cell Fates RegulationsCombinatorial LibrariesPleiotropism

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