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Tissue Contraction Force Microscopy for Optimization of Engineered Cardiac Tissue.

Jeremy A Schaefer¹, Robert T Tranquillo^1,2

¹1 Department of Biomedical Engineering, University of Minnesota-Twin Cities , Minneapolis, Minnesota.

Tissue Engineering. Part C, Methods

|November 6, 2015

Summary

We developed a novel assay to measure cardiac tissue twitch force using fluorescent beads in a PDMS substrate. This cost-effective platform enables rapid screening of cardiac tissue contractility.

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Area of Science:

Biomedical Engineering
Cardiovascular Research
Stem Cell Biology

Background:

Accurate measurement of cardiac tissue contractility is crucial for drug development and disease modeling.
Existing methods for assessing cardiac tissue force can be complex and time-consuming.
Millimeter-scale cardiac tissues offer a relevant model for studying cardiac function.

Purpose of the Study:

To develop and validate a high-throughput screening assay for measuring the twitch force of gel-based cardiac tissues.
To enable relative comparison of cardiac tissue contractility using a simple and cost-effective platform.
To assess the influence of fibrin gel concentration on cardiac tissue contractility.

Main Methods:

Developed a high-throughput assay utilizing traction force microscopy principles with fluorescent microspheres in a polydimethylsiloxane (PDMS) substrate.
Created hemispherical cardiac tissue samples by pipetting cell suspensions onto the PDMS substrate.
Recorded fluorescent bead movement during tissue pacing to quantify substrate displacement and infer twitch force.

Main Results:

Bead displacement amplitude correlated well with direct force measurements, validating the assay's utility.
Cardiac tissues in 2 mg/mL and 4 mg/mL fibrin gels exhibited similar twitch forces, despite visual differences in free-floating samples.
The assay demonstrated rapid sample preparation, data collection, and analysis.

Conclusions:

The developed assay is a valuable tool for high-throughput screening of cardiac tissue contractility.
This platform provides a simple, cost-effective, and rapid method for assessing cardiac tissue function.
The assay's ability to differentiate contractility independent of visual cues highlights its precision.