Related Experiment Video

Updated: Mar 30, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.9K

MicroRNA-340 Mediates Metabolic Shift in Oral Squamous Cell Carcinoma by Targeting Glucose Transporter-1

Ping Xu1, Yan Li2, Haiyuan Zhang2

  • 1Attending Physician, Department of Stomatology, Chengdu Military General Hospital, Chengdu, China.

Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons
|November 7, 2015
PubMed
Abstract

Insights

MicroRNA-340 (miR-340) downregulation promotes oral cancer growth by increasing glucose metabolism. This metabolic shift, driven by elevated glucose transporter-1 (Glut1), fuels rapid oral squamous cell carcinoma (OSCC) proliferation.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • MicroRNA-340 (miR-340) is frequently altered in human cancers, impacting tumor progression.
  • MicroRNAs are increasingly recognized for their roles in regulating cellular energy metabolism.
  • The specific metabolic contributions of miR-340 in oral squamous cell carcinoma (OSCC) remain underexplored.

Purpose of the Study:

  • To investigate the role of miR-340 in the metabolic reprogramming of oral squamous cell carcinoma (OSCC).
  • To elucidate the relationship between miR-340 expression and key metabolic pathways in OSCC.

Main Methods:

  • Quantitative assessment of miR-340 expression using real-time polymerase chain reaction.
  • Functional analysis of miR-340 mimics and inhibitors in SAS human tongue SCC cells, evaluating proliferation, colony formation, lactate secretion, and glucose uptake.
  • Investigation of the correlation between miR-340 levels and glucose transporter-1 (Glut1) expression.

Main Results:

  • Reduced miR-340 expression was observed in oral cancer cells, correlating with a metabolic shift.
  • Downregulation of miR-340 led to increased glucose transporter-1 (Glut1) expression.
  • Elevated Glut1 expression resulted in heightened lactate secretion and glucose uptake rates, promoting rapid cancer cell proliferation.

Conclusions:

  • MiR-340 functions as a critical regulator of glycolysis in OSCC.
  • The regulatory mechanism involves modulating glucose transporter-1 (Glut1) expression.
  • MiR-340's influence on glycolysis and Glut1 presents a potential therapeutic target for OSCC.

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K