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T-lymphocyte clones responsive to Shigella flexneri
S H Zwillich1, A D Duby, P E Lipsky
1Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas 75235.
Journal of Clinical Microbiology
|March 1, 1989
Summary
Researchers isolated T-lymphocyte clones from a patient with Shigella dysentery and reactive arthritis. These clones proliferated in response to Shigella, indicating a potential role in post-infectious immune responses.
Area of Science:
- Immunology
- Microbiology
- Gastroenterology
Background:
- Shigella flexneri dysentery can lead to reactive arthritis.
- Understanding the T-cell response is crucial for elucidating post-dysenteric complications.
Purpose of the Study:
- To isolate and characterize T-lymphocyte clones from a patient with Shigella dysentery and reactive arthritis.
- To investigate the proliferative response of these T-cells to Shigella antigens.
Main Methods:
- Cloning of T lymphocytes by limiting dilution using recombinant interleukin-2.
- Proliferation assays using Shigella strains and antigen-presenting cells.
- Phenotypic characterization of T-cell clones (CD3, CD4, CD8) and HLA typing.
Main Results:
- Five of eight T-cell clones proliferated in response to Shigella.
- One clone (MC12) showed specific proliferation to infecting and generating Shigella strains.
- MC12 was identified as CD4+ and HLA-DR+, with responses restricted by HLA-DR2 and blocked by anti-HLA-DR antibodies.
- The recognized epitope was associated with bacterial membranes.
Conclusions:
- T-lymphocyte clones that proliferate in response to Shigella can be isolated from patients with shigellosis.
- These findings contribute to understanding the T-cell-mediated immune response in reactive arthritis following Shigella infection.