Probiotic Strains Influence on Infant Microbiota in the In Vitro Colonic Fermentation Model GIS1

Area of Science:

• Microbiology
• Gastroenterology
• Food Science

Background:

• Lactic acid bacteria (LAB) are crucial for gut health.
• Understanding LAB's interaction with the human colon microbiota is vital for developing effective probiotics.
• The GIS1 system provides an in vitro model for simulating the human colon environment.

Purpose of the Study:

• To evaluate the individual effects of five lyophilized LAB strains on simulated human colon microbiota.
• To assess the impact of these LAB strains on metabolic activity, specifically protein and polysaccharide synthesis.
• To identify LAB strains with significant probiotic potential for industrial applications.

Main Methods:

• Utilized the GIS1 system for in vitro simulation of human colon microbiota.
• Administered five specific lyophilized LAB strains individually: Lactobacillus fermentum 428ST, Lactobacillus rhamnosus E4.2, Lactobacillus plantarum FCA3, Lactobacillus sp. 34.1, and Weissella paramesenteroides FT1a.
• Quantitatively determined protein and polysaccharide levels in each colon segment to assess metabolic activity.

Main Results:

• Lactobacillus rhamnosus E4.2 and Weissella paramesenteroides FT1a demonstrated enhanced exopolysaccharide synthesis.
• These two strains also exhibited superior probiotic potential compared to the others tested.
• Metabolic activity, indicated by protein and polysaccharide production, varied among the tested LAB strains.

Conclusions:

• Lactobacillus rhamnosus E4.2 and Weissella paramesenteroides FT1a are highly effective in exopolysaccharide production within the simulated colon environment.
• These strains possess significant probiotic potential, making them suitable candidates for development.
• The findings support the recommendation of L. rhamnosus E4.2 and W. paramesenteroides FT1a for use in probiotic products and the food industry.