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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Mar 30, 2026

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

Super-Resolution Microscopy: From Single Molecules to Supramolecular Assemblies.

Andrew M Sydor¹, Kirk J Czymmek², Elias M Puchner³

¹Cell Biology Program, The Hospital for Sick Children, Toronto, Canada; Department of Biochemistry, University of Toronto, Toronto, Canada.

Trends in Cell Biology

|November 8, 2015

Summary

This summary is machine-generated.

Super-resolution microscopy (SRM) reveals nanoscale cellular structures, mapping protein complexes and quantifying macromolecule organization. This review guides researchers in applying SRM for advanced biological insights.

Keywords:

cell architecture electron microscopy nanoscopy organelle protein complexes quantitative super-resolution super-resolution microscopy

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Last Updated: Mar 30, 2026

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

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Published on: January 21, 2013

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

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Published on: January 6, 2026

Area of Science:

Cellular and Molecular Biology
Biophysics
Microscopy

Background:

Traditional light microscopy is limited by diffraction, hindering nanoscale visualization.
Super-resolution microscopy (SRM) overcomes diffraction limits for nanometer-scale biological imaging.
Understanding macromolecular organization in vivo is crucial for cell biology.

Purpose of the Study:

To review the applications of SRM in studying macromolecular structures within the native cellular environment.
To highlight SRM's capability in generating molecular maps and quantitative data.
To provide guidance on experimental considerations for SRM studies.

Main Methods:

Survey of various Super-resolution microscopy techniques.
Analysis of SRM's role in visualizing protein complexes and macromolecules.
Discussion of quantitative data extraction from SRM images.

Main Results:

SRM enables detailed molecular mapping of protein complexes.
Quantitative data on macromolecule number, size, and distribution can be obtained.
Novel insights into spatial organization of cellular components are revealed.

Conclusions:

SRM is a powerful tool for elucidating biological structures at the nanoscale.
This review serves as a guide for researchers utilizing SRM in biological sciences.
SRM facilitates a deeper understanding of cellular architecture and function.