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[Establishment of a SCID beige mouse model bearing transplanted human choriocarcinoma using JAR cell line]
Objective:
To establish a mouse model bearing human choriocarcinoma xenograft in severe combined immuno-deficient (SCID) beige mice and investigate the disease course and biological behaviors of the tumors.
Methods:
Human choriocarcinoma JAR cells were injected in female SCID beige mice (3-5 weeks old) either subcutaneously (group A, n=6) or via the tail vein (group B, n=6). Morphological studies, radioactive immunoassay, in vivo tumor imaging and histopathological examinations were performed to confirm JAR cell engraftment at the subcutaneous injection site and in the lungs of the mice.
Results:
On day 28 after tumor cell inoculation, the mice in group A showed palpable subcutaneous nodules, and HE staining revealed morphological features of the nodules consistent with choriocarcinoma cells; in vivo imaging in group B showed single or multiple solid tumor masses in the lungs, and tissue biopsy examination demonstrated varying degrees of tumor cell infiltration. Compared with the control mice, peripheral blood β-HCG levels in both groups A and B increased significantly on day 14 after cell inoculation (P<0.05), and the increment was more conspicuous in group B (P<0.05).
Conclusion:
Mouse models bearing human choriocarcinoma xenograft can be successfully established by injecting JAR cells either subcutaneously or via the tail vein to mimic the characteristics of epithelial solid tumors and lung metastasis of human choriocarcinoma.
Insights
This study successfully created mouse models of human choriocarcinoma xenografts using JAR cells. These models mimic epithelial solid tumors and lung metastasis, aiding choriocarcinoma research.
Area of Science:
- Oncology
- Immunology
- Animal Models
Background:
- Human choriocarcinoma is a rare but aggressive placental tumor.
- Establishing reliable animal models is crucial for understanding choriocarcinoma progression and metastasis.
- Severe combined immunodeficient (SCID) beige mice are commonly used for xenograft studies due to their impaired immune system.
Purpose of the Study:
- To develop a SCID beige mouse model of human choriocarcinoma xenograft.
- To investigate the disease course and biological behaviors of human choriocarcinoma tumors in vivo.
- To evaluate the potential of subcutaneous and intravenous injection routes for xenograft establishment.
Main Methods:
- Human choriocarcinoma JAR cells were injected subcutaneously (Group A) or intravenously via the tail vein (Group B) into female SCID beige mice.
- Tumor engraftment was confirmed using morphological studies, radioactive immunoassay, in vivo imaging, and histopathological examinations.
- Peripheral blood beta-human chorionic gonadotropin (β-HCG) levels were monitored as a biomarker.
Main Results:
- Subcutaneous xenografts (Group A) showed palpable nodules with choriocarcinoma morphology by day 28.
- Intravenous injection (Group B) resulted in lung tumor masses and infiltration, confirmed by imaging and biopsy.
- Both groups exhibited significantly increased peripheral blood β-HCG levels from day 14, with a more pronounced increase in the intravenous group.
Conclusions:
- Successful establishment of human choriocarcinoma xenograft mouse models is achievable via both subcutaneous and tail vein injection of JAR cells.
- These models effectively mimic the characteristics of epithelial solid tumors and lung metastasis observed in human choriocarcinoma.
- The developed models provide a valuable platform for studying choriocarcinoma biology and evaluating therapeutic strategies.
