Jove
Visualize
Contact Us

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Continuing Medical Education as a Tool to Accelerate the Adoption of Extended-Duration Intravitreal Anti-VEGFs.

Journal of CME·2026
Same author

Improving follow-up survey completion rates through pilot interventions in the All of Us Research Program: Results from a non-randomized intervention study.

PloS one·2024
Same author

Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors.

Pflugers Archiv : European journal of physiology·2012
Same author

Galphas-biased beta2-adrenergic receptor signaling from restoring synchronous contraction in the failing heart.

Science translational medicine·2011
Same author

Myocardial injury after ischemia-reperfusion in mice deficient in Akt2 is associated with increased cardiac macrophage density.

American journal of physiology. Heart and circulatory physiology·2011
Same author

PI3K/Akt signaling requires spatial compartmentalization in plasma membrane microdomains.

Proceedings of the National Academy of Sciences of the United States of America·2011
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 30, 2026

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

9.1K

Visualization of Compartmentalized Kinase Activity Dynamics Using Adaptable BimKARs.

Charlene Depry1, Sohum Mehta2, Ruojing Li1

  • 1Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Chemistry & Biology
|November 10, 2015
PubMed
Summary

Bimolecular kinase activity reporters (bimKARs) offer enhanced real-time visualization of kinase activity dynamics. These versatile tools reveal complex signaling regulation, such as protein kinase A

Keywords:
AMPKFRETPKAbiosensorcompartmentalized signalinglive-cell imaging

More Related Videos

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

8.4K
Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

14.8K

Related Experiment Videos

Last Updated: Mar 30, 2026

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

9.1K
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

8.4K
Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

14.8K

Area of Science:

  • Cellular signaling and kinase activity monitoring
  • Molecular imaging and biosensor development

Background:

  • Real-time monitoring of kinase activity is crucial for understanding spatiotemporal regulation of cellular signaling.
  • Existing methods may lack the dynamic range to capture subtle changes in kinase activity.

Purpose of the Study:

  • To report on the adaptability and utility of bimolecular kinase activity reporters (bimKARs) for live-cell imaging.
  • To demonstrate the enhanced dynamic range of bimKARs compared to unimolecular reporters.
  • To investigate the regulation of AMP-activated protein kinase (AMPK) by protein kinase A (PKA) at the plasma membrane.

Main Methods:

  • Development and application of bimolecular kinase activity reporters (bimKARs).
  • Monitoring of kinase activity for JNK, ERK, and AMPK in live cells.
  • Investigation of AMPK regulation by PKA using bimKARs.

Main Results:

  • bimKARs demonstrated versatility in monitoring various kinases, including JNK, ERK, and AMPK.
  • bimKARs exhibited significantly enhanced dynamic ranges compared to unimolecular reporters.
  • The study revealed dual regulatory roles (negative and positive) of PKA on AMPK activity within the same cell at the plasma membrane.

Conclusions:

  • bimKARs are adaptable and powerful molecular tools for real-time visualization of kinase activity dynamics.
  • The enhanced dynamic range of bimKARs allows for the detection of previously unresolvable signaling events.
  • PKA exhibits complex, context-dependent regulation of AMPK activity at the plasma membrane.