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Updated: Mar 30, 2026

Induction and Testing of Hypoxia in Cell Culture
Published on: August 12, 2011
Dynamic regulation of stem cell specification and maintenance by hypoxia-inducible factors
1McKusick-Nathans Institute of Genetic Medicine; Institute for Cell Engineering; and Departments of Pediatrics, Medicine, Oncology, Radiation Oncology, and Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Abstract:
Stem cells are characterized by the capacity for both self-renewal and generation of all other cell types (pluripotency) or differentiated cells within a particular lineage (multipotency). Stem cells are often localized to hypoxic niches within tissues and hypoxia inducible factors (HIFs) play key roles in the maintenance of pluripotent and multipotent stem cells, as well as cancer stem cells, which are also known as tumor-initiating cells. HIF inhibitors target cancer stem cells and improve the responses to angiogenesis inhibitors and cytotoxic chemotherapy in mouse models of breast cancer.
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