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Updated: Mar 30, 2026
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
Expansion of CD14(+)CD16(+) monocytes is related to acute leukemia
Xin-Quan Jiang1, Lei Zhang2, Hong-Ai Liu3
1Institute of Public Health, Taishan Medical University No.619 Changcheng Road, Taian, Shandong, People's Republic of China.
Objective:
Aim to investigate the proportion of CD14(+)CD16(+) monocytes and understand the pathogenesis of this monocyte subset in acute leukemia.
Methods:
Flow cytometry was utilized to study the phenotype expression of CD14(+)CD16(+) monocytes and CD3(+) T lymphocytes in peripheral blood derived from patients with acute leukemia. All the data were analyzed by SPSS 13.0 software.
Results:
The proportion of CD14(+)CD16(+) monocytes including both intermediate and non-classical monocytes, increased significantly in patients with acute leukemia and changed negatively or positively according to the disease process. Meanwhile, the proportion of CD14(+)CD16(+) monocytes was inversely correlated with absolute number of CD4(+) T lymphocytes, ratio of CD4(+)/CD8(+) T cells, and positively correlated with the proportion of neutrophil granulocytes.
Conclusions:
The proportion of CD14(+)CD16(+) monocytes (especially the intermediate subpopulation) is related to the progression of acute leukemia, and the expansion of this monocyte subset could indicate the severity of the disease.
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