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Related Experiment Videos

A new method for sequencing DNA.

A M Maxam, W Gilbert

    Proceedings of the National Academy of Sciences of the United States of America
    |February 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a chemical DNA sequencing method that partially breaks DNA at specific bases. Fragment lengths reveal base positions, enabling DNA sequence determination up to 100 bases.

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    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Determining the precise order of nucleotides in DNA is fundamental to molecular biology.
    • Existing DNA sequencing methods require specific chemical or enzymatic treatments.

    Purpose of the Study:

    • To develop a novel chemical procedure for DNA sequencing.
    • To enable the determination of DNA sequences by selective base cleavage.

    Main Methods:

    • Partially cleaving terminally labeled DNA molecules at specific bases (G, A, C+T, C).
    • Resolving fragment sizes using polyacrylamide gel electrophoresis.
    • Identifying base positions from the pattern of radioactive bands.

    Main Results:

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  • Developed four distinct chemical reactions for preferential DNA cleavage at G, A, C+T, and C.
  • Demonstrated that fragment length analysis by gel electrophoresis allows DNA sequence reading.
  • Achieved sequencing of at least 100 bases from the labeling point.

    • Conclusions:

    • The described chemical DNA sequencing technique is effective for determining nucleotide order.
    • This method provides a new tool for DNA sequence analysis.
    • The technique is capable of sequencing significant lengths of DNA.