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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Capillary Electrophoresis for the Selection of DNA Aptamers Recognizing Activated Protein C
Nasim Shahidi Hamedani1, Jens Müller2
1Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany.
Abstract:
Capillary electrophoresis-based SELEX (CE-SELEX) is an efficient technique for the isolation of aptamers binding to a wide range of target molecules. CE-SELEX has a number of advantages over conventional SELEX procedures such as the selection of aptamers can be performed on non-immobilized targets, usually within a fewer number of selection cycles. Here we describe a complete procedure of CE-SELEX using activated protein C (APC) as the target protein.
