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Published on: August 29, 2014
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Preparation of SELEX Samples for Next-Generation Sequencing.
1Life & Medical Sciences Institute (LIMES), University of Bonn, Gerhard-Domagk-Str. 1, Bonn, 53121, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|November 11, 2015
Summary
Next-generation sequencing (NGS) enables deep sequencing of SELEX libraries due to cost reductions. This study details preparing SELEX samples for Illumina sequencing, including adding barcodes for multiplexing and adapter ligation.
Area of Science:
- Biochemistry
- Genomics
- Molecular Biology
Background:
- Massive whole genome sequencing projects have driven technological advancements and reduced costs.
- Next-generation sequencing (NGS) is now cost-effective for deep sequencing of SELEX libraries.
Purpose of the Study:
- To describe the preparation of SELEX samples for Illumina sequencing.
- To adapt existing whole genome sequencing workflows for SELEX library preparation.
Main Methods:
- Addition of barcode sequences for multiplexing SELEX libraries.
- Adapter ligation for preparing SELEX samples for Illumina sequencing.
- Utilizing established whole genome sequencing workflows.
Main Results:
- Successful preparation of SELEX samples compatible with Illumina sequencing.
- Demonstrated a method for multiplexing SELEX libraries using barcodes.
- Identified and mitigated common pitfalls in adapter ligation for SELEX.
Conclusions:
- NGS technologies, particularly Illumina, are highly suitable for deep sequencing of SELEX libraries.
- The described method provides a robust workflow for preparing SELEX samples for high-throughput sequencing.
- This approach facilitates efficient and cost-effective analysis of SELEX experiments.
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