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Aptamer-Based Enzyme Capture Assay for Measurement of Plasma Thrombin Levels
Jens Müller1, Tobias Becher2, Günter Mayer3
1Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany. jens.mueller@ukb.uni-bonn.de.
Methods in Molecular Biology (Clifton, N.J.)
|November 11, 2015
Abstract:
The quantification of circulating thrombin is a valuable tool to accurately assess the activity of the blood coagulation system. Here, we describe the combined application of the thrombin-specific reversible active-site inhibitor argatroban and the DNA-aptamer HD1-22 for conduction of an enzyme capture assay for reliable measurement of plasma thrombin levels.
