Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Types of Hypothesis Testing01:11

Types of Hypothesis Testing

29.8K
There are three types of hypothesis tests: right-tailed, left-tailed, and two-tailed.
When the null and alternative hypotheses are stated, it is observed that the null hypothesis is a neutral statement against which the alternative hypothesis is tested. The alternative hypothesis is a claim that instead has a certain direction. If the null hypothesis claims that p = 0.5, the alternative hypothesis would be an opposing statement to this and can be put either p > 0.5, p < 0.5, or p...
29.8K
Null and Alternative Hypotheses01:16

Null and Alternative Hypotheses

13.4K
The actual hypothesis testing begins by considering two hypotheses. They are termed  the null hypothesis and the alternative hypothesis. These hypotheses contain opposing viewpoints.
The null hypothesis, denoted by H0 is a statement of no difference between the variables—they are not related. This can often be considered the status quo. As  a result if you cannot accept the null, it requires some action.
The alternative hypothesis, denoted by H1 or Ha, is a claim about the...
13.4K
Test for Homogeneity01:23

Test for Homogeneity

2.5K
The goodness–of–fit test can be used to decide whether a population fits a given distribution, but it will not suffice to decide whether two populations follow the same unknown distribution. A different test, called the test for homogeneity, can be used to conclude whether two populations have the same distribution. To calculate the test statistic for a test for homogeneity, follow the same procedure as with the test of independence. The hypotheses for the test for homogeneity can...
2.5K
Multiple Comparison Tests01:13

Multiple Comparison Tests

4.6K
Multiple comparison test, abbreviated as MCT, is a post hoc analysis generally performed after comparing multiple samples with one or more tests. An MCT will help identify a significantly different sample among multiple samples or a factor among multiple factors.
It would be easy to compare two samples using a significance alpha level of 0.05. In other words, there is only one sample pair to be compared. However, it would be difficult to identify a significantly different sample if the number...
4.6K
Decision Making: Traditional Method01:14

Decision Making: Traditional Method

5.8K
The process of hypothesis testing based on the traditional method includes calculating the critical value, testing the value of the test statistic using the sample data, and interpreting these values.
First, a specific claim about the population parameter is decided based on the research question and is stated in a simple form. Further, an opposing statement to this claim is also stated. These statements can act as null and alternative hypotheses, out of which a null hypothesis would be a...
5.8K
Testing a Claim about Standard Deviation01:19

Testing a Claim about Standard Deviation

3.2K
A complete procedure to test a claim about population standard deviation or population variance is explained here.
The hypothesis testing for the claim of population standard deviation (or variance) requires the data and samples to be random and unbiased. The population distribution also must be normal. There is no specific requirement on the sample size as the estimation is based on the chi-square distribution.
As a first step, the hypothesis (null and alternative) concerning the claim about...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacokinetics in Early Drug Research.

Alternatives to laboratory animals : ATLA·2015
Same author

Transgenic animals: more than "commercial opportunities".

Alternatives to laboratory animals : ATLA·2015
Same author

Comments on appendix C of the national institutes of health response to the petition of the american anti-vivisection society to prohibit the use of animals in the production of monoclonal antibodies.

Alternatives to laboratory animals : ATLA·2015
Same author

A Study on UV Filter Chemicals from Annex VII of European Union Directive 76/768/EEC, in the In Vitro 3T3 NRU Phototoxicity Test.

Alternatives to laboratory animals : ATLA·2015
Same author

An evaluation of the proposed OECD testing strategy for skin corrosion.

Alternatives to laboratory animals : ATLA·2015
Same author

Validation of alternative methods for the potency testing of vaccines.

Alternatives to laboratory animals : ATLA·2015
Same journal

Spotlight on Three Rs Progress.

Alternatives to laboratory animals : ATLA·2026
Same journal

Resources Round-up.

Alternatives to laboratory animals : ATLA·2026
Same journal

Editorial.

Alternatives to laboratory animals : ATLA·2026
Same journal

<i>Galleria mellonella</i> as an invertebrate screening model for biocompatibility and <i>in vivo</i> functionality testing of imaging contrast agents.

Alternatives to laboratory animals : ATLA·2026
Same journal

Data-driven science, ethics and risk: Building knowledge of key ethics-risk intersections relevant to public sector decision-making.

Alternatives to laboratory animals : ATLA·2026
Same journal

Conference Diary.

Alternatives to laboratory animals : ATLA·2026
See all related articles

Related Experiment Video

Updated: Mar 30, 2026

Author Spotlight: Validation of SICOLE-R for Assessing Cognitive and Reading Skills in Spanish-Speaking Children and Its Role in Personalized Education
09:00

Author Spotlight: Validation of SICOLE-R for Assessing Cognitive and Reading Skills in Spanish-Speaking Children and Its Role in Personalized Education

Published on: August 16, 2024

1.3K

Progress Toward the Validation of Alternative Tests.

M Balls1, J H Fentem1

  • 1ECVAM, Ispra, Italy.

Alternatives to Laboratory Animals : ATLA
|November 12, 2015
PubMed
Summary
This summary is machine-generated.

The European Centre for the Validation of Alternative Methods (ECVAM) validates alternative testing methods for regulatory use. This review discusses challenges and solutions in validation, focusing on method development and inter-laboratory transferability.

More Related Videos

Development of a Virtual Reality Assessment of Everyday Living Skills
10:32

Development of a Virtual Reality Assessment of Everyday Living Skills

Published on: April 23, 2014

19.4K
A Computerized Functional Skills Assessment and Training Program Targeting Technology Based Everyday Functional Skills
07:31

A Computerized Functional Skills Assessment and Training Program Targeting Technology Based Everyday Functional Skills

Published on: February 13, 2020

7.5K

Related Experiment Videos

Last Updated: Mar 30, 2026

Author Spotlight: Validation of SICOLE-R for Assessing Cognitive and Reading Skills in Spanish-Speaking Children and Its Role in Personalized Education
09:00

Author Spotlight: Validation of SICOLE-R for Assessing Cognitive and Reading Skills in Spanish-Speaking Children and Its Role in Personalized Education

Published on: August 16, 2024

1.3K
Development of a Virtual Reality Assessment of Everyday Living Skills
10:32

Development of a Virtual Reality Assessment of Everyday Living Skills

Published on: April 23, 2014

19.4K
A Computerized Functional Skills Assessment and Training Program Targeting Technology Based Everyday Functional Skills
07:31

A Computerized Functional Skills Assessment and Training Program Targeting Technology Based Everyday Functional Skills

Published on: February 13, 2020

7.5K

Area of Science:

  • Toxicology
  • Regulatory Science
  • In Vitro Methods

Background:

  • The European Centre for the Validation of Alternative Methods (ECVAM) plays a crucial role in validating alternative methods for regulatory testing.
  • Ensuring the reliability and applicability of these alternative methods is essential for reducing animal testing.

Purpose of the Study:

  • To review ECVAM's role and criteria in validating alternative methods for regulatory testing.
  • To discuss challenges encountered during validation studies and propose solutions.
  • To highlight the importance of method development and prevalidation.

Main Methods:

  • Review of ECVAM's validation criteria and priorities.
  • Discussion of difficulties in validation studies.
  • Emphasis on the ECVAM prevalidation scheme for protocol optimization and inter-laboratory transferability assessment.
  • Analysis of challenges in selecting test materials with adequate in vivo data and conducting in vivo/in vitro comparisons.

Main Results:

  • Identified key criteria for prioritizing validation studies.
  • Proposed solutions for common difficulties in validation, including protocol optimization and inter-laboratory transferability.
  • Highlighted issues with test material selection and the need for robust in vivo/in vitro comparisons.

Conclusions:

  • Effective validation of alternative methods requires well-developed protocols and robust prevalidation schemes.
  • Addressing challenges in test material selection and in vivo/in vitro comparisons is critical for successful regulatory acceptance of alternative methods.
  • ECVAM's approach aims to enhance the reliability and transferability of alternative testing methods.