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Multispecific lymphoid cell surface receptors.

M J Czaja, F F Richards, J M Varga

    Proceedings of the National Academy of Sciences of the United States of America
    |March 1, 1977
    PubMed
    Summary
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    Mice immunized with two antigens develop "double-binding" lymphocytes. These cells express multispecific immunoglobulin receptors capable of binding both antigens, suggesting a single receptor type for committed antibody production.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Cell Biology

    Background:

    • Sequential immunization in mice can induce oligoclonal antibody responses.
    • Cell surface receptors on lymphocytes play a crucial role in immune responses.
    • Understanding receptor specificity is key to deciphering immune cell function.

    Purpose of the Study:

    • To investigate the nature of cell surface receptors involved in dual-antigen binding.
    • To determine if a single receptor molecule mediates binding to two distinct antigens.
    • To characterize the dynamics of receptor expression during an oligoclonal immune response.

    Main Methods:

    • Sequential immunization of mice with two specific antigens: bovine pancreatic ribonuclease and a 2,4-dinitrophenyl (DNP) derivative.

    Related Experiment Videos

  • Flow cytometry and competition assays to analyze cell surface receptor binding.
  • Analysis of splenic lymphocyte populations.

    • Main Results:

    • Sequential immunization led to an increase in "double-binding" splenic lymphocytes.
    • Competition studies indicated that the same molecular receptors bind both ribonuclease and DNP.
    • Initially, double-binding cells predominated, later diluted by cells binding only DNP.

    Conclusions:

    • Committed antibody-producing cells likely express a predominant multispecific cell surface immunoglobulin receptor.
    • This receptor exhibits dual specificity, binding to both ribonuclease and DNP.
    • The findings provide insights into the molecular basis of immune receptor diversity and specificity.