Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Toxins01:12

Bacterial Toxins

49
Bacterial toxins are sophisticated virulence factors that enable pathogenic bacteria to interact with, invade, and damage host tissues. These toxins fall broadly into two types: protein exotoxins, which are secreted into the environment and target specific host receptors, and lipopolysaccharide endotoxins, which are structural components of the bacterial outer membrane released primarily during bacterial lysis or membrane shedding. Exotoxins generally act more selectively, binding to cell...
49

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell tracking as a tool for studying EMT-phenotypes.

Experimental cell research·2024
Same author

Initial refinement of data from video-based single-cell tracking.

Cancer innovation·2023
Same author

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)<sup>1</sup>.

Autophagy·2021
Same author

Advanced technological tools to study multidrug resistance in cancer.

Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy·2019
Same author

Single-Cell Tracking of A549 Lung Cancer Cells Exposed to a Marine Toxin Reveals Correlations in Pedigree Tree Profiles.

Frontiers in oncology·2018
Same author

Mitotic Catastrophe in BC3H1 Cells following Yessotoxin Exposure.

Frontiers in cell and developmental biology·2017

Related Experiment Video

Updated: Mar 30, 2026

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
14:29

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

Published on: October 1, 2012

12.6K

Lifetime Distributions from Tracking Individual BC3H1 Cells Subjected to Yessotoxin.

Mónica Suárez Korsnes1, Reinert Korsnes2

  • 1Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences , Ås , Norway.

Frontiers in Bioengineering and Biotechnology
|November 12, 2015
PubMed
Summary

This study tracks individual cell behavior after exposure to yessotoxin (YTX), revealing complex cell fates and demonstrating cell tracking

Keywords:
cell deathcell trackinginter-cellular influencelifetime statisticsyessotoxin

More Related Videos

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
15:05

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

Published on: February 5, 2015

9.9K
Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
09:39

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Published on: May 30, 2013

10.6K

Related Experiment Videos

Last Updated: Mar 30, 2026

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
14:29

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

Published on: October 1, 2012

12.6K
Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
15:05

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

Published on: February 5, 2015

9.9K
Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages
09:39

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Published on: May 30, 2013

10.6K

Area of Science:

  • Cell Biology
  • Toxicology
  • Biophysics

Background:

  • Aggregate cell properties can mask individual cell fate complexities.
  • Understanding cellular responses to toxins is crucial for various biological and medical applications.
  • Yessotoxin (YTX) is a marine toxin with known cytotoxic effects.

Purpose of the Study:

  • To demonstrate the complexity of individual cell lifetime distributions after yessotoxin (YTX) exposure.
  • To showcase the general practicality and utility of cell tracking for high-throughput analysis of cytotoxic effects.
  • To explore how individual cell lifetime distributions can reveal different cell death modalities and inter-cellular influences.

Main Methods:

  • Time-lapse microscopy was used to track individual BC3H1 cells.
  • Cell lifetime distributions were analyzed for populations exposed to yessotoxin (YTX).
  • The study focused on analyzing single-cell data to reveal heterogeneity in cell fate.

Main Results:

  • Individual cell lifetime distributions after YTX exposure exhibit complexity.
  • Cell tracking effectively captures individual cell fate, which is often obscured in aggregate data.
  • Observed lifetime distributions suggest the involvement of different cell death modalities.

Conclusions:

  • Cell tracking is a practical, non-intrusive method for high-throughput analysis of cytotoxic effects.
  • Analysis of individual cell lifetime distributions can reveal sub-populations and inter-cellular influences.
  • Findings may contribute to understanding tumor-cell resistance and metastasis probability.