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Related Experiment Videos

Immunophenotyping in a multicenter study: the Transfusion Safety Study experience.

M A Fletcher1, S P Azen, B Adelsberg

  • 1University of Miami School of Medicine, Florida 33101.

Clinical Immunology and Immunopathology
|July 1, 1989
PubMed
Summary
This summary is machine-generated.

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The Transfusion Safety Study (TSS) investigated how HIV-1 impacts immune cells in males with congenital clotting disorders (CCD). HIV-1 infection in older males with CCD led to specific T-cell subset reductions, while younger males showed elevated immunocytes.

Area of Science:

  • Immunology
  • Hematology
  • Virology

Background:

  • The Transfusion Safety Study (TSS) investigates transfusion-transmitted infections.
  • Lymphocyte immunophenotyping via flow cytometry is crucial for assessing immune status.
  • Standardization and quality control are essential for multicenter longitudinal studies.

Purpose of the Study:

  • To assess immune cell profiles in males with congenital clotting disorders (CCD).
  • To compare immune cell changes in HIV-1 positive versus negative individuals within the CCD cohort.
  • To analyze age-related differences in immune cell distribution in CCD patients.

Main Methods:

  • Utilizing two-color flow cytometry with a broad panel of monoclonal antibodies for lymphocyte immunophenotyping.
  • Conducting a multicenter, longitudinal study with standardized instrumentation, reagents, and protocols.

Related Experiment Videos

  • Performing baseline assessments on a cohort of males aged 10 and over with CCD.

    • Main Results:

    • In HIV-1 positive males (≥10 years) with CCD, T4+ cell loss was primarily due to a decrease in the T4+4B4+ subset.
    • HIV-1 positive males (≥10 years) with CCD showed increased CD8+ cells (I2+T8+, Leu7+T8+) but decreased NKH.1+T8+ cells.
    • Younger CCD patients (<10 years), regardless of HIV-1 status, exhibited elevated immunocytes, including CD4+ cells, with a distinct shift in CD4+ subsets (increased 2H4+T4+ relative to 4B4+T4+).

    Conclusions:

    • HIV-1 infection differentially affects T-cell subsets in older males with CCD.
    • Age significantly influences immune cell profiles in individuals with CCD, with younger patients showing distinct immunocyte elevations and subset distributions.
    • Standardized flow cytometry protocols are vital for reliable immune monitoring in multicenter studies like TSS.