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DNA Triplexes That Bind Several Cofactor Molecules.

Sven Vollmer1, Clemens Richert2

  • 1Institut für Organische Chemie, Universität Stuttgart, 70569 Stuttgart (Germany), Fax: (+49) 711-685-64321.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|November 13, 2015
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Summary
This summary is machine-generated.

Synthetic DNA triplexes can capture and release energy-rich biomolecules like ATP. Optimized DNA motifs bind multiple ligands with high affinity, enabling applications like bioluminescence fuel at body temperature.

Keywords:
DNAcofactorsnucleotide storageoligonucleotidestriplexes

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Area of Science:

  • Synthetic biology
  • Biochemistry
  • Materials science

Background:

  • Cofactors are essential for cellular energy processes.
  • Controlling cofactor concentration is key for practical applications.
  • DNA triplexes can capture and release nucleotides.

Purpose of the Study:

  • To enhance the storage capacity of DNA triplex motifs for ligands.
  • To explore the limits of ligand binding in designed DNA cavities.
  • To develop synthetic DNA for high-density capture, storage, and release of biomolecules.

Main Methods:

  • Synthesis of oligonucleotides with non-nucleotide bridges.
  • Measurement of ligand binding (ATP, cAMP, FAD) to triplex motifs.
  • Immobilization of DNA triplexes for functional assays.

Main Results:

  • Triplex motifs with multiple binding sites showed tighter purine binding.
  • An optimized 59-residue triplex with C3-bridges binds four ligands.
  • Apparent dissociation constants (Kd) achieved: ATP (52 µM), FAD (9 µM), cAMP (2 µM).
  • Immobilized triplex fueled bioluminescence via ATP release at body temperature.

Conclusions:

  • Synthetic DNA can be engineered into motifs for high-density biomolecule capture and release.
  • Designed DNA triplexes offer a platform for controlling energy-rich molecules.
  • This technology has potential applications in bioenergetics and diagnostics.