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Polymer Microarrays for High Throughput Discovery of Biomaterials

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Multiplexed MALDI-MS arrays for screening of MIP solid phase extraction materials.

Kishore Kumar Jagadeesan¹, Celina Wierzbicka², Thomas Laurell¹

¹Department of Biomedical Engineering, Lund University, Lund, Sweden.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|November 14, 2015

Summary

This study introduces the Integrated Selective Enrichment Target (ISET) platform for rapid solid phase extraction (SPE) optimization. The ISET system enables efficient sample preparation, saving time and resources in analytical workflows.

Keywords:

ISET MALDI MS Mass spectrometry Proteomics Solid-phase extraction

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Area of Science:

Analytical Chemistry
Biochemistry

Background:

Solid phase extraction (SPE) is crucial for sample preparation but can be time-consuming and resource-intensive.
Optimizing SPE protocols often requires significant amounts of sorbent and sample.
Developing efficient and cost-effective SPE methods is essential for high-throughput analysis.

Purpose of the Study:

To present the microfabricated Integrated Selective Enrichment Target (ISET) platform for rapid and parallel SPE protocol investigation.
To demonstrate the efficiency and economic advantages of the ISET system interfaced with MALDI mass spectrometry.
To showcase the utility of the ISET platform for developing affinity sorbents, specifically molecularly imprinted polymers (MIPs).

Main Methods:

Utilized the ISET platform for high-throughput SPE, processing 96 samples in 2 hours.
Employed small volumes (600 nL) of SPE sorbent per well within the ISET nanovials.
Developed and tested phosphoserine imprinted MIP (pS-MIP) sorbent for phosphopeptide enrichment.

Main Results:

The ISET platform enabled the analysis of over 500 nanovials using only 25mg of pS-MIP sorbent under various conditions.
Multiplexed experiments facilitated the early identification and minimization of unspecific interactions.
Achieved improved enrichment of phosphopeptides through optimized SPE protocols developed on the ISET system.

Conclusions:

The ISET platform offers an efficient, economic, and generic solution for SPE sample preparation optimization.
The ability to use minimal sorbent amounts is advantageous for developing novel affinity sorbents like MIPs.
This technology significantly accelerates the discovery and refinement of SPE protocols, enhancing phosphopeptide enrichment.