Related Experiment Video
Updated: Mar 30, 2026
Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
Determination of nifurtimox in dog plasma by stable-isotope dilution LC-MS/MS
Mark-Jean Gnoth1, Peter-Michael Hopfe1, Uwe Thuss1
1Drug Metabolism & Pharmacokinetics, Pharmaceutical Research Center, Bayer HealthCare AG, Aprather Weg 18A, D-42096 Wuppertal, Germany.
Background:
Nifurtimox is a 5-nitrofuran derived antiprotozoal drug used to treat diseases caused by trypanosomes including Chagas' disease and sleeping sickness (African trypanosomiasis). Available methods for the determination of nifurtimox in plasma are tedious and of low sensitivity. For the first time, an isotope dilution HPLC/MS/MS method for the sensitive quantitation of nifurtimox down to 10.0 μg/l in plasma is described.
Results:
Protein precipitation was used for sample preparation. Samples were analyzed on a standard triple quadrupole tandem mass spectrometer. The validated concentration range covers 10.0 μg/l (LLOQ) to 5000 μg/l. Interassay accuracy and precision (%CV) ranged from 98.4 to 101%, and 2.61 to 10.1%, respectively.
Conclusion:
The method consists of very simple sample preparation and provides unmatched sensitivity, high reproducibility and robustness enabling analysis of large sample numbers. Method performance met current guidelines on bioanalytical method validation.
More Related Videos
08:41Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
10:13Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs
Published on: November 8, 2024