A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor

Area of Science:

• Biotechnology
• Nanomaterials Science
• Molecular Biology

Background:

• Graphene oxide (GO) is a versatile nanomaterial with unique electronic and surface properties.
• Aptamer-based biosensors offer high specificity for target molecule detection.
• Adenosine triphosphate (ATP) is a crucial biomarker in various biological processes.

Purpose of the Study:

• To develop a novel biosensing platform integrating a DNA aptasensor with graphene oxide for ATP detection.
• To achieve rapid, facile, and highly sensitive detection of ATP.
• To explore the potential of GO-DNA nanobiointerfaces in biological sensing and diagnostics.

Main Methods:

• Integration of a GO-locked DNA aptasensor with specific ATP recognition sites and a self-replication track.
• Utilizing differential binding affinities of DNA structures to GO for target-induced release.
• Employing a complementary DNA strand (CPDNA) to facilitate aptasensor release.
• Leveraging polymerization/nicking enzyme synergistic isothermal amplification for signal enhancement.

Main Results:

• The developed biosensor demonstrated rapid and facile detection of ATP.
• Achieved enhanced sensitivity with a wide linear dynamic response range (four orders of magnitude).
• Exhibited good selectivity for ATP detection.
• Successfully demonstrated the GO-DNA nanobiointerface for biological sensing.

Conclusions:

• The novel biosensing platform provides a sensitive and selective method for ATP detection.
• This strategy expands the application of GO-DNA nanobiointerfaces in biological sensing.
• The platform shows significant potential for fundamental research and biomedical diagnosis.