Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

2.0K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
2.0K
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

1.3K
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
1.3K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.8K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.8K
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

2.6K
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
2.6K
Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

4.1K
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
4.1K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

4.1K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Lactobacillus acidophilus</i> protects against <i>Corynebacterium pseudotuberculosis</i> infection by regulating the autophagy of macrophages and maintaining gut microbiota homeostasis in C57BL/6 mice.

mSystems·2024
Same author

Morphology Control of Zr-Based Luminescent Metal-Organic Frameworks for Aflatoxin B1 Detection.

Biosensors·2024
Same author

Achieving a solar-to-chemical efficiency of 3.6% in ambient conditions by inhibiting interlayer charges transport.

Nature communications·2024
Same author

Identifying Factors Affecting the Survival of Patients with HIV-Associated B-Cell Lymphoma Using a Random Survival Forest Model.

Clinical Medicine Insights. Oncology·2024
Same author

Enhancing postoperative analgesia in open orthopedic surgery through acupoint catgut embedding.

Chinese medical journal·2024
Same author

A 14-Bit Hybrid Analog-to-Digital Converter for Infrared Focal Plane Array Digital Readout Integrated Circuit.

Sensors (Basel, Switzerland)·2024

Related Experiment Video

Updated: Mar 30, 2026

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

9.3K

Investigation of the kinetic process of solid phase microextraction in complex sample.

Ruifen Jiang1, Jianqiao Xu1, Wei Lin1

  • 1MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, PR China.

Analytica Chimica Acta
|November 18, 2015
PubMed
Summary

The addition of 2-hydroxypropyl-β-cyclodextrin (β-HPCD) enhances the desorption rates of polyaromatic hydrocarbons (PAHs) from solid-phase microextraction (SPME) fibers in aquatic samples. This study developed a predictive model for these kinetic parameters and analyzed temperature effects.

Keywords:
Complex matrixDepletion methodKinetic processSolid phase microextraction

More Related Videos

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

9.2K
The MPLEx Protocol for Multi-omic Analyses of Soil Samples
10:12

The MPLEx Protocol for Multi-omic Analyses of Soil Samples

Published on: May 30, 2018

11.8K

Related Experiment Videos

Last Updated: Mar 30, 2026

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

9.3K
Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

9.2K
The MPLEx Protocol for Multi-omic Analyses of Soil Samples
10:12

The MPLEx Protocol for Multi-omic Analyses of Soil Samples

Published on: May 30, 2018

11.8K

Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry

Background:

  • Complex matrices in aquatic systems influence the environmental behavior of hydrophobic organic compounds (HOCs).
  • Polyaromatic hydrocarbons (PAHs) are a significant class of HOCs with environmental persistence.
  • Solid-phase microextraction (SPME) is a common technique for analyzing HOCs in water.

Purpose of the Study:

  • To investigate the effect of 2-hydroxypropyl-β-cyclodextrin (β-HPCD) on the desorption kinetics of PAHs from SPME fibers.
  • To develop and validate a theoretical model for predicting PAH desorption enhancement by β-HPCD.
  • To examine the influence of temperature on PAH desorption kinetics and develop a calibration method.

Main Methods:

  • Automated solid-phase microextraction (SPME) with focus on the desorption step.
  • Application of 2-hydroxypropyl-β-cyclodextrin (β-HPCD) to aqueous samples containing PAHs.
  • Development of a theoretical model to describe and predict desorption kinetics.
  • Temperature-dependent kinetic studies following the Arrhenius equation.

Main Results:

  • β-HPCD significantly increased the desorption rates of selected PAHs from the SPME fiber coating.
  • The proposed theoretical model accurately predicted the enhancement effect of β-HPCD on desorption kinetics.
  • Desorption time constants increased with elevated sampling temperatures, consistent with the Arrhenius equation.
  • Higher temperatures increased the lability of HOCs bound within the sample matrix.

Conclusions:

  • β-HPCD is an effective enhancer for PAH desorption from SPME fibers in aquatic environments.
  • The developed theoretical model provides a robust framework for determining kinetic parameters and understanding desorption mechanisms.
  • Temperature plays a crucial role in HOC desorption kinetics, influencing both SPME efficiency and matrix lability.
  • A novel calibration method based on the theoretical model enables accurate analysis of PAHs in complex samples.