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Updated: Mar 30, 2026
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Published on: July 27, 2022
3,3'-Diindolylmethane: A Promising Sensitizer of γ-Irradiation
Wenjing Wang1, Maomin Lv1, Chaoji Huangfu1
1Department of Blood Biopharmaceuticals and Viral Detection, Institute of Transfusion Medicine, The Academy of Military Medical Sciences, Beijing 100850, China.
Purpose:
Radiotherapy is an effective treatment modality in the clinical treatment of breast cancer. The present work investigated the effect of 3,3'-diindolylmethane (DIM) on γ-irradiation sensitizing human breast carcinoma.
Methods:
Cell survival, intracellular ROS levels, cell cycle distribution, cell apoptosis, and expression of proteins related to apoptosis were measured with MTT assays, flow cytometry, and Western blot analysis, respectively.
Results:
In vitro DIM plus γ-irradiation arrested the activity of G2/M phase cell cycle, increased intracellular ROS level, significantly suppressed PARP (poly ADP-ribose polymerase), and enhanced γ-irradiation-induced apoptosis, thereby inhibiting the proliferation of MCF-7 cells.
Conclusion:
These data provide a rationale for the use of DIM as a promising sensitizer of γ-irradiation.
