Related Experiment Video
Updated: Mar 30, 2026
Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
Published on: April 5, 2024
Evaluation of the Proliferative Effects Induced by Low-Level Laser Therapy in Bone Marrow Stem Cell Culture
Marcos Fernando Xisto Braga Cavalcanti1,2, Durvanei A Maria3, Natalia de Isla2
11 Postgraduate Department, Cruzeiro do Sul University São Paulo , SP, Brasil .
Objective:
The objective of this study was to evaluate the effect of laser irradiation on dog bone marrow stem cells.
Background Data:
Low doses of low-level red laser positively affect the viability of mesenchymal stem cells, and also increase proliferation.
Methods:
Low-level laser (wavelength, 660 nm; power output, 50 mW), was applied to dog bone marrow stem cell cultures (DBMSC). The energy densities delivered varied from 1 to 12J/cm(2). The effect of the laser irradiation was evaluated on cell proliferation measured with the MTT colorimetric test, cell cycle phase, and on lipidic peroxidation (free radical production).
Results:
The results indicate that laser irradiation to DBMSC did not change the morphology of the cells, but significantly increased their viability and the number of cells at the G2/M phase with 6, 10, and 12 J/cm(2). On the other hand, malonaldehyde production was significantly enhanced with 8 J/cm(2).
Conclusions:
The parameters used to irradiate DBMSC increased significantly proliferation without producing high levels of reactive oxygen species (ROS).
