Developments in FTICR-MS and Its Potential for Body Fluid Signatures
Simone Nicolardi1, Bogdan Bogdanov2, André M Deelder3
1Center for Proteomics and Metabolomics, Leiden University Medical Center (LUMC), PO Box 9600, 2300 RC Leiden, The Netherlands. s.nicolardi@lumc.nl.
International Journal of Molecular Sciences
|November 19, 2015
Summary
Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) offers ultra-high resolution for complex clinical proteomics. Innovations enable robust FTICR-MS for routine analysis of biomarkers in serum and saliva for disease classification.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Clinical Proteomics
Background:
- Fourier transform mass spectrometry (FTMS) provides ultra-high resolution.
- Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) and Orbitrap™ mass spectrometry have expanded FTMS applications.
- Clinical proteomics requires analyzing complex biological samples like serum and plasma.
Purpose of the Study:
- To review innovations in FTICR-MS for robust, routine analysis of clinical samples.
- To demonstrate the application of FTICR-MS for discovering and validating protein biomarker signatures.
- To highlight FTICR-MS capabilities for cancer disease classification using peptide profiles.
Main Methods:
- Utilizing FTICR-MS for ultra-high resolution mass measurements.
- Employing advanced ionization techniques and chromatographic separation.
- Applying diverse fragmentation strategies for protein characterization, including PTMs.
Main Results:
- FTICR-MS enables analysis of complex samples, simplifying upfront fractionation.
- Achieved accurate and precise mass-to-charge ratio (m/z) measurements for peptides and small proteins.
- Successfully applied FTICR-MS peptide profiles for cancer disease classification.
Conclusions:
- FTICR-MS offers superior resolving power and mass accuracy for clinical proteomics.
- Innovations have led to robust FTICR-MS procedures for routine analysis of clinical samples.
- FTICR-MS is a powerful tool for biomarker discovery and disease classification in serum and saliva.
Keywords:
FTICR-MSclinical cohortinstrumental developmentmass spectrometryprofilingproteomicssalivaserumsolid-phase extractionultra-high resolving powerMore Related Videos
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