Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hallmarks of Cancer: How did it inspire you?

Cell·2026

Same author

WEE1 inhibitors trigger GCN2-mediated activation of the integrated stress response.

Nature communications·2025

Same author

Targeting prohibitins activates the ISR through DELE1-HRI by impairing protein import into the mitochondrial matrix.

Cell death and differentiation·2025

Same author

The mitochondrial disulphide relay substrate FAM136A safeguards IMS proteostasis and cellular fitness.

Redox biology·2025

Same author

Genetic suppression features ABHD18 as a Barth syndrome therapeutic target.

Nature·2025

Same author

AMPylation Regulates 5'-3' Exonuclease PLD3 Processing.

Molecular & cellular proteomics : MCP·2025

Search research articles

Related Experiment Video

Updated: Mar 30, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

A Haploid Genetic Screen Identifies Heparan Sulfate Proteoglycans Supporting Rift Valley Fever Virus Infection.

Amber M Riblett¹, Vincent A Blomen², Lucas T Jae²

¹Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Journal of Virology

|November 20, 2015

Summary

This summary is machine-generated.

Rift Valley fever virus (RVFV) uses glycosaminoglycans (GAGs) for cell attachment, a key step in infection. This discovery in human cells offers new targets for developing antiviral therapies against RVFV.

More Related Videos

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Published on: July 29, 2014

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

Related Experiment Videos

Last Updated: Mar 30, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Published on: July 29, 2014

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

Area of Science:

Virology
Cell Biology
Host-Pathogen Interactions

Background:

Rift Valley fever virus (RVFV) causes significant epizootics and lethal hemorrhagic fever in humans.
RVFV is an emerging pathogen spreading globally, with no approved vaccines or antivirals.
Understanding RVFV's host cell interactions is crucial for developing therapeutics.

Purpose of the Study:

To identify host factors essential for RVFV infection using genetic screening.
To elucidate the role of glycosaminoglycans (GAGs) in RVFV entry.
To inform the development of novel antiviral strategies against RVFV.

Main Methods:

Deep mutagenesis screening in haploid human cells to identify host dependency factors.
Biochemical and genetic approaches to investigate the role of GAGs in RVFV infection.
Examination of other Bunyaviridae family members for GAG-dependent infection.

Main Results:

Genetic screens identified enzymes in GAG biogenesis and transport, including COG complex components, as critical for RVFV infection.
Disruption of PTAR1 conferred RVFV resistance and reduced heparan sulfate levels.
RVFV strains require GAGs for efficient virion attachment to certain cell types, but not all.
GAG-dependent infection is not universal among Bunyaviridae, suggesting alternative attachment mechanisms.

Conclusions:

RVFV utilizes glycosaminoglycans for efficient attachment to host cells, particularly on specific cell types.
The findings highlight GAGs as potential targets for antiviral interventions against RVFV.
Further research is needed to identify additional attachment factors for RVFV and other bunyaviruses.