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DELLA proteins interact with FLC to repress flowering transition
Mingzhe Li1, Fengying An1, Wenyang Li1
1State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
DELLA proteins interact with FLOWERING LOCUS C (FLC) to regulate flowering time. This interaction enhances FLC
Area of Science:
- Plant Biology
- Molecular Genetics
Background:
- Flowering time is crucial for plant reproduction.
- SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and FLOWERING LOCUS T (FT) integrate gibberellic acid (GA) and vernalization pathways.
- DELLA proteins regulate GA signaling, while FLOWERING LOCUS C (FLC) represses SOC1 and FT in vernalization.
Purpose of the Study:
- To elucidate the molecular mechanisms integrating GA and vernalization pathways in flowering time control.
- To investigate the interaction between DELLA proteins and FLC.
Main Methods:
- In vitro and in vivo interaction assays.
- Phenotypic analysis of FLC mutants and overexpressors.
- Gene expression analysis.
Main Results:
- DELLA proteins physically interact with FLC.
- LHRI domains of DELLAs and the MADS domain C-terminus of FLC are essential for interaction.
- FLC modulates GA response in flowering.
- DELLA-FLC interaction enhances FLC's gene repression activity.
Conclusions:
- DELLA and FLC interaction integrates signaling pathways for flowering time control.
- This interaction provides new insights into FLC and DELLA regulatory functions.
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