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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
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DNA Microarray Analysis of Estrogen-Responsive Genes.

Kathleen M Eyster1

  • 1Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, 414 E. Clark St., Vermillion, SD, 57069, USA. Kathleen.Eyster@usd.edu.

Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2015
PubMed
Summary
This summary is machine-generated.

DNA microarray technology enables analysis of thousands of genes simultaneously. This chapter details its use for studying differential gene expression changes induced by estrogen treatment.

Keywords:
DNA microarrayEstrogenGene expression

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • DNA microarray is a powerful discovery technology for analyzing gene expression.
  • It allows simultaneous assessment of thousands of genes, offering a comprehensive view of cellular activity.
  • Applications include identifying differential gene expression, genetic mutations, DNA methylation, and microRNA expression.

Purpose of the Study:

  • To describe DNA microarray technology.
  • To explain its application in analyzing differential gene expression.
  • Specifically focusing on gene expression changes in response to estrogen treatment.

Main Methods:

  • Utilizes DNA microarray platforms for high-throughput gene expression analysis.
  • Involves hybridizing labeled nucleic acids to a solid surface containing known DNA sequences.
  • Quantifies the abundance of specific transcripts to infer gene activity levels.

Main Results:

  • Demonstrates the capability of DNA microarrays to detect significant changes in gene expression.
  • Highlights the identification of specific genes modulated by estrogen treatment.
  • Provides a basis for understanding estrogen-mediated biological pathways.

Conclusions:

  • DNA microarray technology is a versatile tool for gene expression profiling.
  • It is effective in identifying molecular responses to stimuli like estrogen.
  • This approach facilitates the discovery of disease-related genetic alterations and therapeutic targets.