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DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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The concept of the looking-glass self describes how an individual's self-concept is shaped by their perception of how others see them. This psychological theory, first introduced by sociologist Charles Horton Cooley in 1902, posits that self-identity emerges in a social context and is influenced by the judgments—real or imagined—of others.Research suggests that individuals frequently overestimate how positively others perceive them. This is particularly evident in physical...
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When designing a water slide, controlling the speed of water flow is crucial for rider safety while maintaining an exciting experience. As water flows down the slide, gravity causes it to accelerate, with its speed at the bottom depending on the height from which it starts. The higher the slide, the more potential energy the water has at the top, which is converted into kinetic energy as it descends, increasing its speed.
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DNA Helicases00:55

DNA Helicases

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DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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Translesion DNA Polymerases02:10

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Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
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DNA Topoisomerases02:02

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Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
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ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
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Protocol for DNA Microarrays on Glass Slides.

Kathleen M Eyster1

  • 1Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD, USA. Kathleen.Eyster@usd.edu.

Methods in Molecular Biology (Clifton, N.J.)
|May 23, 2019
PubMed
Summary
This summary is machine-generated.

DNA microarrays offer a flexible method for discovering gene expression patterns. This technology enables the analysis of gene expression, long noncoding RNAs, and specific pathologies using DNA sequences on slides.

Keywords:
DNA microarrayGene expressionRNA analysisRNA extraction

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • DNA microarrays are versatile tools for biological discovery.
  • They enable the assessment of various biological processes, including gene expression and disease pathology.
  • This technology utilizes DNA sequences immobilized on microscope slides.

Purpose of the Study:

  • To describe the protocol for DNA microarray analysis.
  • To detail the methodology for assessing differential gene expression.
  • To provide a guide for utilizing DNA microarrays in research.

Main Methods:

  • Utilizing DNA sequences spotted on microscope slides.
  • Implementing a protocol for DNA microarray analysis.
  • Analyzing differential gene expression through microarray hybridization.

Main Results:

  • Demonstrated the application of DNA microarrays for gene expression profiling.
  • Successfully applied microarray technology to identify differential gene expression.
  • Provided a reproducible protocol for DNA microarray analysis.

Conclusions:

  • DNA microarrays are powerful, flexible, and nonbiased discovery tools.
  • The described protocol facilitates the analysis of differential gene expression.
  • Microarray technology is applicable to a wide range of biological investigations.