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Regulation of Activation Induced Deaminase (AID) by Estrogen

Siim Pauklin1

  • 1Anne McLaren Laboratory for Regenerative Medicine, Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, CB2 0SZ, UK. sp579@cam.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2015
PubMed

Regulation of Activation Induced Deaminase (AID) by the hormone estrogen has important implications for understanding adaptive immune responses as well as the involvement of AID in autoimmune diseases and tumorigenesis. This chapter describes the general laboratory techniques for analyzing AID expression and activity induced by estrogen, focusing on the isolation and preparation of cells for hormone treatment and the subsequent analysis of AID responsiveness to estrogen at the RNA level and for determining the regulation of AID activity via estrogen by analyzing Ig switch circle transcripts and mutations in switch region loci.

Keywords:
AIDActivation induced deaminaseAdaptive immune response sAutoimmune disease sEstrogenIg switch circle transcript sSomatic hypermutation sSplenic B-cell s

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