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Updated: Aug 9, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
Published on: July 26, 2010
The dual aptamer approach: rational design of a high-affinity FAD aptamer
T Merkle1, I T Holder, J S Hartig
1Department of Chemistry and Konstanz Research School Chemical Biology (KoRS-CB), University of Konstanz, Universitätsstr. 10, 78457 Konstanz, Germany. joerg.hartig@uni-konstanz.de.
Abstract:
A design strategy for high-affinity aptamers of complex biomolecules is presented. We developed an RNA with FAD-binding properties by combining known ATP- and FMN-aptamers. Cooperative binding of FAD was shown by SPR spectroscopy and fluorescence assays. The strategy should be transferable to several other biomolecules.
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