Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

81.3K
Overview
81.3K
CRISPR01:59

CRISPR

59.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
59.1K
RNA Editing02:23

RNA Editing

10.2K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
10.2K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.6K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.6K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.6K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biofabrication of synthetic human liver tissue with advanced programmable functions.

iScience·2022
Same author

Hepatocyte Nuclear Factor 4 alpha 2 Messenger RNA Reprograms Liver-Enriched Transcription Factors and Functional Proteins in End-Stage Cirrhotic Human Hepatocytes.

Hepatology communications·2021
Same author

Cellular Location of HNF4α is Linked With Terminal Liver Failure in Humans.

Hepatology communications·2020
Same author

Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells.

Cell reports·2020
Same author

[Emergence of 3D human fatty liver models engineered in the laboratory].

Medecine sciences : M/S·2020
Same author

Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism.

Cell metabolism·2019

Related Experiment Video

Updated: Mar 29, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.3K

From hacking the human genome to editing organs.

Takamasa Tobita1, Jorge Guzman-Lepe1, Alexandra Collin de l'Hortet1

  • 1a Department of Pathology ; University of Pittsburgh ; Pittsburgh ; PA USA.

Organogenesis
|November 21, 2015
PubMed
Summary

Human genome engineering tools like CRISPR have rapidly advanced, offering new possibilities for personalized medicine and biotechnology. This review covers recent progress, applications, and ethical considerations in human gene editing.

Keywords:
CRISPRTALENZFNgene editinggenome engineeringorgan edition

More Related Videos

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
07:19

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

Published on: February 18, 2020

19.3K
Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

4.7K

Related Experiment Videos

Last Updated: Mar 29, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.3K
Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
07:19

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

Published on: February 18, 2020

19.3K
Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

4.7K

Area of Science:

  • Biotechnology
  • Genetics
  • Molecular Biology

Background:

  • Human genome engineering is a rapidly evolving field with significant implications for medicine and biotechnology.
  • The development of engineered nucleases, including Zinc Finger Nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), and CRISPR, has accelerated progress.

Purpose of the Study:

  • To review recent advances in human genome engineering tools.
  • To discuss the implications of these technologies in research and medicine.
  • To address the limitations and ethical concerns associated with human gene editing.

Main Methods:

  • Literature review of recent advances in genome engineering technologies.
  • Analysis of the applications and implications of ZFNs, TALENs, and CRISPR.
  • Discussion of ethical considerations and future directions in the field.

Main Results:

  • Significant progress has been made in developing and refining genome engineering tools.
  • These tools offer broad applications in research, personalized medicine, and biotechnology.
  • The rapid evolution of these technologies necessitates careful consideration of their limitations and ethical concerns.

Conclusions:

  • Engineered nucleases have revolutionized human genome engineering, opening new avenues for medical and biotechnological innovation.
  • Continued research and open discussion are crucial to responsibly harness the potential of gene editing technologies.
  • Addressing the ethical, legal, and social implications is paramount for the safe and effective application of human genome engineering.