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Related Experiment Video

Updated: Mar 29, 2026

In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses
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In situ microscopy using adjustment-free optics.

Hajo Suhr1, Alois M Herkommer2

  • 1Hochschule Mannheim-University of Applied Sciences, Department of Information Technology, Paul-Wittsack-Straße 10, D-68163 Mannheim, Germany.

Journal of Biomedical Optics
|November 22, 2015
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Summary
This summary is machine-generated.

This study introduces a novel, high-resolution in situ microscope (ISM) with a rigid construction, eliminating the need for continuous focusing. This innovation enables unattended operation for monitoring microparticles in agitated suspensions with submicron resolution.

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Area of Science:

  • Microscopy
  • Particle Analysis
  • Biotechnology

Background:

  • In situ microscopy (ISM) monitors microparticles in agitated suspensions.
  • Current ISMs require constant focusing, limiting unattended applications.
  • High-resolution imaging of dynamic microparticle systems is challenging.

Purpose of the Study:

  • To develop a high-resolution in situ microscope (ISM) for unattended operation.
  • To overcome the focusing limitations of existing ISM techniques.
  • To enable precise monitoring of microparticles in agitated suspensions.

Main Methods:

  • Designed a rigid-construction ISM, eliminating the need for adjustments.
  • Utilized a specially designed water immersion objective (NA=0.75, WD=15 μm).
  • The objective interfaces directly with the moving suspension, using off-the-shelf parts.

Main Results:

  • Achieved high-resolution imaging without manual or automated focusing.
  • Demonstrated submicron resolution within moving suspensions.
  • Successfully imaged microorganisms in agitated environments.

Conclusions:

  • The developed rigid-construction ISM enables high-resolution, unattended monitoring.
  • This technology expands the application of ISMs as reliable sensors.
  • Facilitates advanced studies of microparticle dynamics in various fields.