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Updated: Mar 29, 2026

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
Published on: March 14, 2011
Roles of natural killer cells in antiviral immunity
Stephen N Waggoner1, Seth D Reighard2, Ivayla E Gyurova3
1Center for Autoimmune Genomics and Etiology (CAGE), Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States; Immunology Graduate Program, University of Cincinnati, Cincinnati, OH, United States; Medical Scientist Training Program, University of Cincinnati, Cincinnati, OH, United States; Pathobiology and Molecular Medicine Graduate Program, University of Cincinnati, Cincinnati, OH, United States.
Abstract:
Natural killer (NK) cells are important in immune defense against virus infections. This is predominantly considered a function of rapid, innate NK-cell killing of virus-infected cells. However, NK cells also prime other immune cells through the release of interferon gamma (IFN-γ) and other cytokines. Additionally, NK cells share features with long-lived adaptive immune cells and can impact disease pathogenesis through the inhibition of adaptive immune responses by virus-specific T and B cells. The relative contributions of these diverse and conflicting functions of NK cells in humans are poorly defined and likely context-dependent, thereby complicating the development of therapeutic interventions. Here we focus on the contributions of NK cells to disease in diverse virus infections germane to human health.
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